ProteoSure™ Endoproteinase GluC, V8 Protease, for Proteomic Sequencing and Mass Spectrometry Analysis

Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues. The Optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors include Phosphoric acid diisopropyl ester fluoride (DFP), alpha-2-macroglobulin, and Nα-Tosyl-L-lysine chloromethyl ketone (TLCK). Endoproteinase Glu-C is active in the presence of 0.5% SDS and retains 80% activity after 12 hours at 25°C. It also remains its 95% enzymatic activity after 2 hours in 2 M urea. Endoproteinase Glu-C is suitable for peptide sequencing and mapping applications, and protein identification by peptide mass fingerprinting or MS/MS spectral matching. Instruction to use Recommended condition for digestion: pH 8.0~8.5. Product Details CAS # 66676-43-5 Enzyme Commission # 3.4.21.19 Specific activity Approx. 20 U/mg at 25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate; Approx. 500 U/mg at 37°C with casein as the substrate. Storage -20°C Intended use For research only. Not intended for any human or animal diagnostic or therapeutic use.   Documents Product Information Sheet 🗎    Recombinant V8 Protease, Endoproteinase GluC Technical Note 🗎    Conversion of Enzyme Activity Measurement Units