Entamoeba Ag

The test is based on the immunological capture of colored microparticles as they move along a membrane on which the monoclonal antibody has been immobilized. Entamoeba Ag Rapid Test (RAP-5405 and RAP-5406) use monoclonal antibodies specific for Entamoeba histolytica that detect all forms of the parasite during its life cycle. The test is based on the use of red microspheres covalently linked to an anti-Entamoeba histolytica monoclonal antibody, plus blue microspheres as test control. The parasites present in stool samples react with the latex particles which are coated with specific monoclonal antibodies against the antigen. This latex particles/antibodies/parasite complex migrates through a chromatographic process towards the reaction area. In this area, anti-Entamoeba histolytica antibodies that react with the latex particles/antibodies/parasite complex are present. This reaction leads to the appearance of a red line. These lines are used to interpret the result after a five-minute incubation at room temperature.