ProteoSure™ Recombinant Human Trypsin

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin lies between 7.8 ~ 8.7. The enzyme is inhibited by serine protease inhibitors (e.g. PMSF) and by metal chelating agents (e.g., EDTA). Recombinant Human Trypsin is a genetically engineered protein, expressed in E.coli and purified by high pressure liquid chromatography. The amino acid sequence is identical to Human Trypsin 2. There is no contaminating enzyme activity from carboxypeptidase A or chymotrypsin. No protease inhibitors such as PMSF are used during the manufacturing process.   Product Highlights Animal-origin free The use of recombinant Human Trypsin eliminates the potential risk of contamination with virus and any other potential adventitious agents found in animal-derived trypsin. Recombinant Human Trypsin is 100% AOF, and is manufactured in ISO9001 certified facility. Human recombinant This product is a recombinant human trypsin. The amino acid sequence is identical to Human Trypsin 2. Suitable for applications that require low immunogenic ingredients. Stable Recombinant human trypsin is supplied as sterile lyophilized powder. It minimizes the risk of contamination and activity loss during the process of transport and storage. Highly pure Recombinant human trypsin provides super specificity and purity. No other contaminating proteases such as chymotrypsin or carboxypeptidase A. Purity is greater than 95% by HPLC.   Product Details CAS # 9002-07-07 Enzyme Commission # 3.4.21.4 Molecular weight 23.8 kDa Origin of species Human Source E.coli Purified by HPLC Physical form White or white-like lyophilized powder Specific activity ≥ 2500 USP unit/mg protein Unit definition One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0 mL at pH7.6 and 25℃, with BAEE as a substrate (1 cm light path). Purity ≥ 95% by HPLC Contaminant activity No chymotrypsin, carboxypeptidase A, or other protease contaminants. Storage Store in a sealed container at 2 - 8°C. The product is stable for 24 months. Once reconstituted, it should be stored at -20°C. It is stable for 24 months and greater than 90% enzymatic activity remains after 10 repeated freezing-and-thawing. Intended use For research or processing only. Not intended for any human or animal diagnostic or therapeutic use.   Documents Product Information Sheet 🗎    Recombinant Human Trypsin, 100% Animal Original Free Technical Note 🗎    Conversion of Enzyme Activity Measurement Units