ProteoSure™ Recombinant Protein A, Acid-, Alkali-stable

Protein A from Staphylococcus aureus is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses with high affinity, including human IgGs (IgG1, IgG2, and IgG4); mouse IgGs (IgG2, IgG2a, IgG2b, and IgG3). It also binds to total IgG forms of cow, guinea pig, hamster, house, pig, and rabbit. Protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Protein A can also be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers), for detection of antibodies in the process of Western-blot, ELISA or immunohistochemical tests. ProteoSure™ recombinant Protein A is a genetically engineered mutant variant. It contains IgG-binding domains, having other non-specific binding sequences removed, and carries mutations that contribute to its stability in extreme alkali conditions. This recombinant Protein A has shown improved dynamic binding capacity in the purification of immunoglobulins, and is suitable for cleaning-in-place (CIP) applications using up to 0.5 M NaOH. Manufactured according to NSF ISO9001:2008.   Product Details CAS # 91932-65-9 Molecular weight 25 kDa by 12% SDS-PAGE Origin of species Staphylococcus aureus Source E.coli Physical form Lyophilized powder pH stability range pH 1- 13, stable in 0.5 M NaOH and 0.5 M HCl conditions Purity One major band after electrophoresis on 12% SDS-PAGE UV Spectrum  E275/250 ≥1.2, E0.1% at 275nm=0.17-0.23 Impurity No Triton contaminant; no DNA or RNA detected; no microbial content detected in bioburden test. Storage Store at 2 - 8°C. Intended use For research and processing only. Not intended for any human or animal diagnostic or therapeutic use.   Documents Product Information Sheet 🗎    Protein A, Acid-, Alkaline-tolerant recombinant Material Safety Data Sheet 🗎    Protein A, Acid-, Alkaline-tolerant recombinant Technical Note 🗎    Comparison of Binding Characteristics of Immunoglobulin-Binding Proteins