Recombinant Human GDNF Protein_C230355

GDNF is a disulfide-linked homodimeric neurotrophic factor that is a potent growth factor for dopamine and motor neurons and is structurally related to Artemin, Neurturin, and Persephin.GDNF signals through a multicomponent receptor system consisting of RET and one of the four GFRα (α1-α4) receptors.GDNF specifically promotes dopamine uptake and survival, as well as morphological differentiation of midbrain neurons. In a mouse model of Parkinson's disease, GDNF improves bradykinesia, rigidity and postural instability.This recombinant mouse GDNF is supplied as a lyophilized powder with high activity, high purity, low endotoxin, and no redundant labeling.   Product Properties Synonyms Astrocyte-derived trophic factor, ATF, ATF-1, ATF2, GDNF, HFB1-GDNF, HGDNF, HSCR3 Accession P39905 Source E.coli-derived Human GDNF protein, Ser78-Ile211. Molecular Weight Approximately 31.1 kDa. AA Sequence SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCR PIAFDDD LSFLDDNLVY HILRKHSAKR CGCI Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97 % by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml,corresponding to a specific activity of > 5.0×107 IU/mg. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 µm filtered solution in 1×PBS, pH 7.4, with 0.05 % Tween-20. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.   Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!