PVDF Transfer Membrane

NuSep PVDF Membrane is ideal for chemiluminescent and colorimetric western blotting: it retains target protein very strongly during protein transfer but also reduces nonspecific protein binding that can obscure high-sensitivity detection. Features and Benefits Mechanical strength of PVDF membrane enables multiple reprobing of blots Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background Available in three formats: Convenient precut membrane/blot absorbent filter paper sandwiches Packs of precut membrane-only sheets Economical rolls Applications Transferring proteins from SDS-PAGE gels to membrane Immunoblotting target proteins Detection by western blotting chemiluminescent or colorimetric methods Advantages High binding affinity Low background Ready-to-use pre-cut membrane/filter paper sandwiches Two pore sizes available: 0.45 µm --- most blotting applications 0.20 µm --- lower molecular weights 10 pieces per package for more information