ProteoSure™ Recombinant Endoproteinase Lys-C, for Proteomic Sequencing and Mass Spectrometry Analysis

Endoproteinase Lys-C is a serine protease specific for cleavage of amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues. The recombinant Endoproteinase Lys-C is genetically engineered from the Achromobacter lyticus lysyl endoproteinase. It is expressed in E.coli and purified by high pressure liquid chromatography (HPLC).  Recommended usage The recommended ratio to the sample protein is 1:1000 to 1:20000 (w/w), the optimum pH is pH 7 - 9.5. The enzymatic reaction should be carried out at 30 - 37°C for 2 - 24 hours. The use of enzyme inhibitors (such as aprotinin, DFP, leupeptin, and TLCK) or protein denaturation with guanidine chloride (GuHCl) is not recommended.  Recombinant Lys-C is compatible with the following agents: 0.2% SDS, 1.0% Triton, 6 M Urea, 0.5 M NaCl, and 20% Acetonitrile. Storage and stability Store Lys-C lyophilized powder in a sealed container at 2-8°C. It is stable for 12 months. The lyophilized powder can be reconstituted with ddH2O to the working concentration; and the solution is stable at 2-8°C for 7 days. It is also recommended to dissolve the lyophilized powder in 25 mM Tris-HCl (pH 8.5) and make a stock concentrate solution. Aliquots of the stock solution may be stored at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles. Product Details CAS # 78642-25-8 Enzyme Commission # 3.4.21.50 Molecular weight 31 kDa Origin Achromobacter lyticus Source E.coli Purified by HPLC Physical form Lyophilized powder Specific activity ≥ 3.0 AU/mg protein Unit definition One Amidase Unit (AU) is the amount of enzyme that hydrolyzes 1 micromole Ac-Lys-pNA per minute at 25 mM Tris-HCl (pH 9.0) at 37°C. Contaminant activity No trypsin, chymotrypsin, carboxypeptidase A, Lys-N, Arg-C or other protease contaminants. Storage Store in a sealed container at 2 - 8°C. Once reconstituted, it should be stored at -20°C for up to 6 months. Avoid freeze-and-thaw cycles.   Intended use For research only. Not intended for any human or animal diagnostic or therapeutic use. Documents Technical Note 🗎    Conversion of Enzyme Activity Measurement Units