PROTEINDEX™ Recombinant Protein A Prepacked Gravity Flow Columns, 1 mL, Pack of 5 Columns

PROTEINDEX™ recombinant Protein A Agarose Gravity Flow Column is ready-to-use affinity chromatography column for purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins. Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. The recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in E. coli, and it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit. Enabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications. Each gravity column contains 1 mL of recombinant Protein A agarose resin, and is individually wrapped.   Product Highlights High capacity, > 40 mg human IgG per column High selectivity for high purity Minimal ligand leakage, can be reused High purity and high capacity of recombinant Protein A ligand. 2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE. Lanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.   Product Details Product package size 5 columns Column volume 1 mL settled Protein A resins Ligand Recombinant protein A produced in E.coli Binding capacity >40 mg rabbit IgG Matrix 4% agarose Particle size 45 μm - 165 μm Maximum pressure 0.1 MPa (1 bar) Chemical stability All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea. Recommended flow rate Gravity flow, 100 - 300 cm/hour pH stability Long term: pH 3 - 9; Short term: pH 2 - 10 Storage temperature 2 - 8°C Storage buffer 20% ethanol Column material polypropylene (body) Low density polyethylene (top snap cap and bottom tip cap) Frit Polyethylene, with an average pore size of 30 μm   Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.          Documents Technical Note 🗎    Comparison of Binding Characteristics of Immunoglobulin-Binding Proteins