PROTEINDEX™ Ni-IDA Agarose 6FF

PROTEINDEX™ Ni-IDA Agarose 6FF is designed mainly for scaling up purification of histidine-tagged proteins that are expressed in various hosts, such as E.coli, yeast, insect cells, and mammalian cells. Highly cross-linked 6% agarose as the base matrix support offers excellent mechanical stability for centrifugation, and automated, scale up purification of histidine-tagged proteins.   Product Highlights Mechanical stable, designed for high yield, scaling up purification of histidine-tagged proteins Higher binding capacity Low leakage of nickel ions Versatile, ready for gravity/batch, spin, or FPLC systems   Product Details Ligand Nickel Binding capacity >40 mg 6xHis-tagged protein/mL medium Matrix 6% highly cross-linked agarose supplied as 50% slurry Particle size 45 μm - 165 μm Maximum pressure 0.3 MPa (3 bar) Storage temperature 2 - 8°C Storage buffer 1x PBS containing 20% ethanol   Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not freeze.