PROTEINDEX™ NHS-Activated Agarose 4 Fast Flow

PROTEINDEX™ NHS-Activated Agarose 4 Fast Flow is a pre-activated agarose matrix that provides a versatile coupling chemistry for immobilizing amino-containing proteins and peptides. The pre-activated functional N-hydroxysuccinimide (NHS) group forms a chemically stable amide bone with the ligand that contains a primary amino group. therefore NHS-Activated Agarose 4 Fast Flow can be used to prepare customized affinity purification resin for isolating specific substances from complex mixtures, often achieving very high purity in a single step. The base matrix of the resin is highly cross-linked 4% agarose, and is ready for application on the automated system, spinning on the centrifuge, and operation under the vacuum.   Product Details Ligand N-hydroxysuccinimide (NHS) activated Coupling capacity > 10 mg IgG/mL medium Matrix Highly cross-linked 4% agarose supplied as a 50% slurry Particle size 45 μm - 165 μm, with an average of ~ 90 μm Maximum pressure 0.3 MPa (3 bar) Recommended flow rate Gravity flow and medium pressure, 30 - 300 cm/hour Chemical stability (after coupling) All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea (ligand dependent) pH stability (after coupling) pH 2 – 13 (ligand dependent) Cleaning-in-place (after coupling) 0.1 M ~ 0.5 M NaOH (ligand dependent) Storage temperature 2 - 8°C Storage buffer 100% isopropanol Storage buffer (after coupling) 1x PBS with 20% ethanol   Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C.