PROTEINDEX™ Glutathione Agarose 4 Fast Flow

PROTEINDEX™ Glutathione Agarose 4FF is an affinity chromatography medium developed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect and mammalian cells. The rigid, highly cross-linked support is suitable for purification scale-up applications. Our proprietary ProteIndex™ coupling technology ensures stable immobilization of active ligand and minimal ligand leakage in a wide range of purification buffer conditions. The resins can be reused multiple times to purify the same target protein.   Product Highlights High selectivity for high purity > 10 mg GST/mL settled resins Stable coupling of ligand, low leakage; can be re-used to purify the same protein Versatile, ready-to-use with convenient formats Purification performance of Glutathione 4FF resins. 50 mL of E.coli lysate containing a GST-tagged fusion protein was loaded on 0.5 mL packed Glutathione 4FF resins at 0.5 mL/mL. The column was then washed with 10 mL of PBS. The target GST-tagged protein was eluted with 7.5 mL of elution buffer (50 mM Tris-HCl, 10 mM GSH, pH 8.0). Fractions of lysate, flow-through, and eluate were analysed by SDS-PAGE. Lanes: (1) Raw cell lysate; (2) Flow-through; (3) Eluate.   Product Details Ligand Glutathione coupled with a 12-atom linker Ligand concentration 120 - 320 μmol Glutathione/mL medium Binding capacity >10 mg GST (40 kDa)/mL medium Matrix 4% highly cross-linked agarose supplied as 50% slurry Particle size 45 μm - 165 μm Maximum pressure 0.3 MPa (3 bar, 43 psi) Recommended flow rate 300 - 500 cm/hour pH stability 3-12 Storage temperature 2 - 8°C Storage buffer 20% ethanol as preservative   Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.