Phi29 DNA polymerase (10 U/μL)_N210508

phi29 DNA polymerase is derived from the Bacillus subtilis bacteriophage. After genetic engineering, it exhibits excellent strand displacement and processive synthesis capabilities, enabling the synthesis of DNA fragments up to 70 kb in length. Additionally, it possesses strong 3’→5’ exonuclease proofreading activity. Primer 3’ end modifications are recommended in the recommended reaction system to prevent degradation. This enzyme is commonly used in in vitro plasmid synthesis and whole genome amplification. Specifications Cat.No. N210508S / N210508M / N210508L Size 250 U / 1000 U / 5000 U Unit Definition The amount of enzyme required to incorporate 0.5 pmol of dNTP into acid-insoluble material in 10 minutes at 30°C. Heat Inactivation 65°C for 10 min. Components Components No. Name N210508S N210508M N210508L N210508-A phi29 DNA Polymerase (10 U/μL） 25 μL 100 μL 500 μL N210508-B 10×phi29 Reaction Buffer 100 μL 400 μL 2×1 mL Storage This product should be stored at -25~-15℃ for 2 years. Documents： Manual