NGS rRNA Depletion Kit (Plant) _ N210401

Product description NGS rRNA Depletion Kit (Plant) utilizes RNase H digestion to remove ribosomal RNA (including cytoplasmic 18S, 25S rRNA, mitochondrial 18S, 26S rRNA, and chloroplast 16S, 23S rRNA) from total RNA of plant origin, thereby preserving messenger RNA (mRNA) and other non-coding RNA. This kit provides effective rRNA removal for both intact and partially degraded total RNA samples. The RNA samples obtained after rRNA depletion can be used for high-throughput sequencing to analyze mRNA and non-coding RNA, significantly increasing the proportion of usable sequencing data. They can also be used for cDNA synthesis or other downstream applications. Application Suitable for total RNA samples from various plant sources ranging from 100 ng to 1 μg; suitable for both intact and partially degraded RNA samples. Specifications Cat.No. N210401E / N210401S / N210401M Size 12 T / 24 T / 96 T Components Components No. Name N210401E N210401S N210401M N210401-A Hybridization Buffer 36 μL 72 μL 288 μL N210401-B Probe Mix (Plant) 36 μL 72μL 288 μL N210401-C RNase H Buffer 36 μL 72 μL 288 μL N210401-D Thermostable RNase H 24 μL 48 μL 192 μL N210401-E DNase I Buffer 330 μL 660 μL 2×1320 μL N210401-F DNase I 30 μL 60 μL 240 μL Storage Shipped on dry ice, store at -25~-15 °C.Valid for one year. Notes 1. Please use RNase-free consumables and regularly clean the work area. Thermo Fisher Scientific’s RNAZap™ High-Efficiency Nuclease Removal Spray is recommended for RNase contamination removal. 2. RNA samples should be free of genomic DNA contamination. If gDNA is present, perform DNase I digestion followed by purification before using this kit. 3. The maximum input volume for RNA samples is 10 μL. If the sample volume exceeds this, concentrate the RNA before use. 4. If the RNase H digestion step requires a reaction mix, please prepare it freshly before use. 5. For personal safety and health, wear a lab coat and disposable gloves during operation. 6. This product is for research use only. Documents： Manual