NGS Total RNA Library Prep Kit_N210102

NGS Total RNA Library Prep Kit is a total RNA sequencing library preparation kit compatible with both Illumina and MGI platforms. It includes reagents for RNA fragmentation, reverse transcription, conventional and strand-specific double-stranded cDNA (ds-cDNA) synthesis, and library amplification. It can be used in conjunction with mRNA purification kits or rRNA depletion kits to prepare sequencing libraries. The second-strand synthesis module contains two types of buffers, allowing users to choose between conventional or strand-specific library preparation. In the strand-specific second-strand synthesis buffer, dTTP is replaced with dUTP, enabling dUTP incorporation into the second cDNA strand. The high-fidelity DNA polymerase used in this kit cannot amplify DNA templates containing uracil, thereby achieving strand specificity. All reagents included in the kit have undergone rigorous quality control and functional validation to ensure maximum library preparation stability and reproducibility. Specifications Cat.No. N210102S / N210102M Size 24 T / 96 T Components Components No. Name N210102S N210102M N210102-A 2× Frag/Prime Buffe 250 μL 930 μL N210102-B 1st Strand Enzyme Mix 48 μL 192 μL N210102-C Strand Specificity Reagent 150 μL 580 μL N210102-D 2nd Strand Buffer (dNTP) 720 μL 2×1440 μL N210102-E 2nd Strand Buffer (dUTP) 720 μL 2×1440 μL N210102-F 2nd Strand Enzyme Master Mix 120 μL 480 μL N210102-G Ligation Enhancer 720 μL 2×1440 μL N210102-H Novel T4 DNA Ligase 120 μL 480 μL N210102-I 2 × Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL N210102-K Nuclease Free H2O 300 μL 1000 μL N210102-* Primer mix NA NA 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual