NGS DNA Library Prep Kit (Enzymatic)_N210001

The NGS DNA Library Prep Kit (Enzymatic) is a next-generation enzymatic library preparation kit compatible with both IlluminaTM and MGITM high-throughput sequencing platforms. Compared to traditional methods, this kit eliminates the need for cumbersome sonication by using high-quality fragmentation enzymes. The fragmentation and end-repair with dA-tailing modules are combined into a single step, significantly reducing the time and cost of library preparation. It is suitable for input amounts ranging from 1 ng to 1 μg of standard plant and animal genomic DNA, microbial genomic DNA, etc. The enzyme produces uniform fragmentation across different species with minimal species-specific variation. DNA fragmentation, end-repair and dA-tailing are all performed in a single tube. The kit is compatible with both IlluminaTM and MGITM adapters and primers for sequencing on both platforms. Applicable to genomic DNA and full-length cDNA input amounts of 1 ng to 1 μg High-quality fragmentation enzyme enables random dsDNA cleavage with minimal bias Fragmentation, end repair, and dA-tailing in a single step High-fidelity enzyme with strong amplification efficiency significantly improves library quality and yield Suitable for a wide range of species Strict batch performance and stability control Specifications Cat.No. N210001E / N210001S / N210001M /N210001L Size 8 T / 24 T / 96 T /96 T(Plate-Packaging） Components Components No. Name N210001E N210001S N210001M N210001L* N210001-A SmearaseTM Buffer 4.0 80 μL 240 μL 960 μL 8×150 μL N210001-B SmearaseTM Enzyme 4.0 80 μL 240 μL 960 μL 8×150 μL N210001-C Ligation Enhancer 4.0 240 μL 720 μL 3×960 μL 8×420 μL N210001-D Rapid DNA Ligase 4.0 80 μL 240 μL 2×480 μL 8×140 μL N210001-E 2×Ultima HF Amplification Mix 200 μL 600 μL 3×800 μL 8×340 μL 【Note】: When using incomplete adapters, no primer mix is required. However, for full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. *For plate format (automated 96T), refer to the layout diagram below:      Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual Related Blog: Enzymatic Fragmentation DNA Library Prep Kits: Empowering Multi-Disciplinary Research Applications