12000bp DNA Marker, 100-12000bp

Product description This product contains a DNA molecular weight marker consisting of 20 linear double-stranded DNA fragments at the following sizes: 12,000 bp; 8,000 bp; 6,000 bp; 5,000 bp; 4,000 bp; 3,000 bp; 2,500 bp; 2,000 bp; 1,500 bp; 1,200 bp; 1,000 bp; 900 bp; 800 bp; 700 bp; 600 bp; 500 bp; 400 bp; 300bp; 200 bp; 100 bp. The reference bands are 500 bp, 1,000 bp and 1,500 bp, with a concentration of 60 ng/5 μL, while all other bands are at 20 ng/5 μL. The marker is supplied in 1×DNA Loading Buffer and is designed for agarose gel electrophoresis analysis of DNA bands. It is not recommended for polyacrylamide gel electrophoresis (PAGE).   Specifications Product No. N132120S N132120M Size 100 T 10×100 T Components Component No.  Component Name N132120S N132120M N132120-A 12 kb DNA Marker 500 μL 10×500 μL N132120-B 5×DNA Loading Buffer 1 mL 10×1 mL Shipping and Storage Store at room temperatures or at 2°C to 8°C, valid for half a year. Stor at -25℃ to -15℃, valid for one year. Avoid repeated freeze-thaw cycles. Notes 1. For optimal electrophoresis results: 1) Ensure thorough mixing of the product before use. 2) Replace the electrophoresis buffer promptly and use freshly prepared gels. 2. If smearing, blurred bands, or distortion occurs during electrophoresis: Dilute the sample with water before loading. For standard-width gel wells, dilute the sample 5-fold with water and load 8-10 μL. 3. When switching to a new stain or using agarose gels containing different stains: 1) Thoroughly clean the electrophoresis tank to avoid cross-contamination. 2) Replace with fresh electrophoresis buffer after cleaning. 4. For your safety and health, please wear a lab coat and disposable gloves. 5. For research use only！ Instructions 1. Load 5 μL of the DNA ladder. For wide wells, increase the loading volume appropriately. 2. Use 0.7-1.2% agarose gels with a voltage of 4–10 V/cm in 0.5×TBE buffer or 1×TAE buffer. 3. Visualize DNA bands under UV light if stain the gel using solution-based staining methods with ethidium bromide (EB) or Arcegen Nucleic Acid Stain (Cat# N132109, non-toxic and UV-compatible).