MWCO Ultrafiltration Spin Units, 12 mL, Pack of 24

Ultrafiltration spin units offer a simple, one step procedure for desalting and concentration of proteins and other macromolecules with greater than 90% recovery. Utilizing molecular weight cut-off polyethersulfone (PES) filter membrane, the spin device is able to retain macromolecules having the greater MW, and filtrate salts and other molecules of the smaller MW. Because of low protein/DNA binding properties of the PES filter, loss of the retentate on the filter is minimized. The retentate can be recovered at excellent rate. Four molecular weight cut-off (MWCO) sizes are provided to cover a broad range of molecular weights: 10 K, 30 K, 50 K, and 100 K. It is recommended to choose an MWCO 1/2 to 1/3 smaller than the protein to be concentrated. For instance, to concentrate a protein of 80 kDa, we recommend to start with the 30 K MWCO spin unit.   Product Highlights Simple, rapid spin for simultaneous desalting and concentrating (up to 75-fold concentration); Buffer exchange in two quick steps; Faster filtration - high flux PES membrane is heat-sealed to the housing unit; High recovery of the retentate - the PES membrane has very low absorption property for protein and DNA/RNA and the filter housing unit is also constructed with low-binding materials; Feasibility of collecting the filtrate - it is flexible to choose either the retentate or the filtrate to keep; Optimized designs for better performance: Built-in dead stop pocket at the filter unit bottom - no risk of dryout during spin; Vertical position of the filter membrane - less clogging/fouling issue; Less processing time, less buffers, and less mess compared to dialysis; Flexible throughput - it is easy to perform multiple samples in parallel or to scale up with the same sample; Free of detectable DNase/RNase contamination.   Applications: Concentration, desalting of proteins, enzymes, antibodies, DNA/RNA and other macromolecules Rapid removal of small contaminants (salts, dyes, detergent, and additives) Post-modification removal of labeled amino acids and nucleotides, or other unincorporated labels Sample pre-treatment prior to HPLC Deproteinization Recovery of biomolecules from cell culture supernatants and cell lysates Concentrating virus from cell culture supernatants Technical Notes 1. Typical retention of protein markers Marker (conc.) MW MWCO Spin time (min) % Retention BSA (1.0 mg/mL) 67 kDa 30 K 30 > 80 % BSA (1.0 mg/mL) 67 kDa 50 K 10 > 80 % Cytochrome C (0.25mg/mL) 12.4 kDa 50 K 10 < 5 % IgG (1.0 mg/mL) 156 kDa 100 K 20 > 80% Ovalbumin (1.0 mg/mL) 45 kDa 100 K 20 < 12%   Single use only. For research use only. Not intended for any human or animal therapeutic or diagnostic uses.   Product Details Maximum sample capacity 12 mL with a swinging-bucket rotor 10 mL with a Fixed-angle rotor (Min. rotor angle is 25°) Material of construction Screw cap: High-density Polyethylene (HDPE) Sealing gasket: Polyethylene (PE) Filtrate receiver tube: Polypropylene (PP) Filter unit: Methyl methacrylate-Butadiene-Styrene (MBS) Ultrafiltration membrane: Polyethersulfone (PES) Active ultrafiltration membrane area 5.48 cm2 Product dimension Assembled unit height: 119 mm Assembled unit O.D.: 29.3 mm Filter unit height: 72.0 mm Filter unit O.D. : 29.5 mm Wetted part No DNase/RNase No detectable DNase/RNase contamination Maxi. centrifugation speed Swinging-bucket: 4,000xg Fixed angle: 5,000xg Working temperature -20°C to 50°C Chemical stability Stable in most commonly used reagents for protein and nucleic acid applications Chemical incompatibility Acetic Acid (≥ 25%) Acetonitrile (≥ 30%) Aliphatic & aromatic esters Amines Ammonium hydroxide (≥ 5%) Aromatic & chlorinated hydrocarbons Butyl acetate (≥ 40%) Dimethyl acetamide (DMAC) (≥ 30%) Ethers Hydrochloric acid (≥ 0.5 N at 50°C) Isopropyl Alcohol (≥ 25%) Ketone Methylene chloride (≥ 1%) Methyl ethyl ketone (≥ 1%) Phosphoric acid (≥ 1 N) Sodium deoxycholate (≥ 5%) Sodium hydroxide (≥ 0.5N at 50°C) Sodium hypochlorite (≥ 0.04%) Tetrahydrofuran (≥ 5%) Toluene (≥ 1%) Sterility Non-sterile Autoclavability No Quality assurance Manufactured in accordance with ISO 13485 : 2016 & ISO 9001 : 2015 quality management systems. Package size 24 spin units per pack. Storage Room temperature. Keep dry. Application note Single use only. For research use only. Not intended for any human or animal therapeutic or diagnostic uses.