ProteoSure™ Recombinant Carboxypeptidase B

Carboxypeptidase B is an enzyme that catalyzes the hydrolysis of basic amino acids —lysine, arginine, and histidine — from the C-terminal end of polypeptides. It has a calculated molecular weight of 34 kDa, an optimal pH of 8.0, and an isoelectric point (pI) of 6.0. The enzyme is competitively inhibited by arginine and lysine, and is also inhibited by citric acid and metal-chelating agents such as EDTA. Marvelgent's recombinant Carboxypeptidase B is expressed in E.coli and is purified by high pressure liquid chromatography. No trace contamination with proteases, such as carboxypeptidase A or chymotrypsin, nor protease inhibitors such as PMSF.   Product Highlights 100% Animal Origin Free    By using of Marvelgent’s recombinant carboxypeptidases B, it helps to avoid contamination with viruses and any other potential adventitious agents that can be found in animal-derived carboxypeptidase B. High purity Recombinant carboxypeptidase B exhibits highly specific enzymatic activity with no contaminating proteases , such as chymotrypsin or carboxypeptidase A that are commonly found in animal-originated enzymes. Stability Provided as sterile lyophilized powder, highly stable during transportation and storage.   Product Details CAS # 9025-24-5 Enzyme Commission # 3.4.17.2 Molecular weight 34.5 kDa Origin of species Rat Source E.coli Purified by HPLC Physical form White to off-white lyophilized powder Additives Tris salts, NaCl and carbohydrates. Protein content 35%～60% Specific activity ≥170 Units/mg protein Unit definition One unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65. Purity > 95% Contaminant activity No chymotrypsin, carboxypeptidase A, or other protease contaminants. Less than 10 ppm of recombinant trypsin. Storage Store in a sealed container at 2 - 8°C. Once reconstituted, it should be stored at -20°C. The product is stable for 24 months. Greater than 90% enzymatic activity retains after 10 repeated freeze-and-thaw cycles. Intended use For research or further processing only. Not intended for any human or animal diagnostic or therapeutic use.   Documents Product Information Sheet 🗎    Recombinant Carboxypeptidase B, 100% Animal Original Free Technical Note 🗎    Conversion of Enzyme Activity Measurement Units